烈性酒中酒精浓度检测方案(定氮仪)

APPLICATION NOTEF&F-K-001-2016/A1 ALCOHOLIC STRENGTH IN SPIRITS DRINKS Alcoholic Strength in Spirits Drinks Reference： COMMISSION REGULATION (EC)N°2870/2000 Tested with VELP Scientifica UDK 129 Kjeldahl Distillation Unit (Code F30200120) Introduction A spirit， or liquor， is an alcoholic distilled beverage containing ethanol， produced by distilling it. Ethanol derives from thefermentation of grain， fruit， or vegetables and adding different aromatic compounds. Spirits drinks are legally consumedin most countries with over 100 countries having laws regulating their production， sale， and consumption. The ethylic alcohol， produced during the fermentation， determines the alcoholic strength and it can be measured byvolume as “the number of liters of ethanol contained in 100 liters of spirits， both volumes being measured at temperatureof 20C. It is expressed by the symbol%vol”. Alcoholic Determination in Spirits Drinks Steam distillation is a method to determine the alcoholic strength in spirits drinks： the distillate obtained is an ethanol-water mixture and the measurement is performed by a digital density meter. Sample Vodka infused with natural flavors Alcoholic strength by volume (labeled value)： 35% vol American Whiskey Alcoholic strength by volume (labeled value)： 40% vol Equipment -Alcoholic strength kit (code A00000285) composed by： -Tweezer for closing the NaOH tube -Kjeldahl balloon 500 ml for the distillation of sample (Code A00000082) -Digital density meter (i.e. Rudolph model DDM 2911 PLUS) -Cooled Incubator (VELP Scientifica FOC Series i.e. code F10400320) -200 ml volumetric flasks with stopper Procedure Measure out 200 ml of the spirits drinks using a graduated flask and thermostat it at 20°C. Let the sample adjust totemperature， this will take about 15 minutes*. Bring down the volume of the sample exactly 200 ml by taking awayexcess sample by a small pipette. Transfer the liquids to the 500 ml test tube. Rinse the volumetric flask with distilled water (3 x 10ml) in order to collect all the mixture residues and add 10 ml ofdistilled water as receiving solution in the same volumetric flask. Place the volumetric flask in a beaker filled with coldwater and ice. Insert the distillate outlet tube (it must be put in contact with the receiving solution) and fix it well usingparafilm. Distillation Collect the distillate in the 200 ml graduated flask used to measure the sample quantity. Preheat the UDK 129 performing a wash down (about 7 minutes). Start the distillation according to the parameters below： e NaOH： 0 ml** Sample volume： 200 ml Sample tube： 500 ml Distillation time ： 7-9 minutes ** Close the NaOH tube using the tweezer received inside the Alcoholic strength kit. In UDK 129 settings， set a distillation time to obtain a maximum of 200 ml of distillate. After the distillation， position thereceiving flask in the thermostat for about 15 min and finally bring up to volume (200 ml) using distilled water at 20 °C. Measure the density of the distillate by the electronic density meter. For diluted samples the result should be doubled. * The samples having a alcohol concentration higher than 25v/v% need to diluted 1：1. Typical Results on Vodka infused with natural flavors *Average±SD% 35.14±0.28 RSD% ** 0.80 Typical Results on American Whiskey *Average±SD% 40.310±0.15 RSD% ** 0.38 *Run of three samples **RSD% =(Standard Deviation x 100) / Average For the verification of the distillation apparatus， the Reg. EC 2870/2000 specifies that， distilling 200 ml of an ethanolwater mixture with an alcoholic strength of 50% vol.， the loss of alcohol must be smaller than 0，1% vol.The UDK 129 satisfies completely the Reg. EC 2870/2000. Conclusion The obtained results are reliable and reproducible in accordance with the expected values， with a low relative standarddeviation (RSD< 1%)， that means high repeatability of the results. Benefits of UDK 129 are： ● High level of precision and reproducibility High productivity Reliable and easy method Time saving ● Affordable equipment cost Moderate running costs spirit(烈酒)是一种含有乙醇的酒精蒸馏饮料，通过蒸馏产生。乙醇是谷物、水果或蔬菜发酵后加入不同的芳香化合物而产生的。烈酒饮料在大多数国家是合法消费的，超过100个国家有法律规范其生产、销售和消费。在发酵过程中产生的乙醇决定了酒精的强度，它可以用体积来测量，即100升白酒中所含乙醇的升数，两者的体积都是在20℃下测量的，用符号%vol表示。检测样品：Vodka infused with natural flavors（有天然风味的伏特加）Alcoholic strength by volume (labeled value)（酒精强度按体积）: 35% volAmerican Whiskey （美国威士忌 ）          Alcoholic strength by volume (labeled value)（酒精强度按体积）: 40% vol：检测设备：意大利velp 凯氏定氮仪UDK129参考标准：COMMISSION REGULATION (EC)N° 2870/2000检测结果：用电子密度计测量蒸馏物的密度。对于稀释的样品，结果应加倍，酒精浓度高于25v/v%的样品需按1:1稀释。  注入天然风味的伏特加的典型结果（三次平均值）：  美国威士忌的典型结果（三次平均值）：RSD% =(标准差x 100) /平均值结论：所得结果可靠，可重现性符合期望值，相对标准偏差低(RSD < 1%)，结果重复性好。UDK 129的好处是:•高水平的精度和再现性•高生产率•方法简便可靠•节约时间•可承受的设备成本•运行成本适中