蛋白质中二级结构的(SSE)程序检测方案

Application Note sc CD-0008 Secondary structure estimation (SSE) program of multiple samples Introduction The multivariate SSE program can be used to analyze sets of CD data. A large number of samples can beautomatically measured and efficiently analyzed using the high-throughput circular dichroism (HDX) systemand multivariate SSE program. Keywords： Secondary structure estimation， Multiple samples， HTCD， HDX Sequence Optical constants are automatically calculated in the multivariate SSE program after imputing parametersas the path length and mean residue molar concentration. 1.63 1 58 Fig. 1 Specifying path length and mean residue molar concentration s.c CD-0008 CD Spectra Measurement 20C Pathlength：1 mmConcentration： 0.2 mg/mlTemp.：Wavelength：260-185 nmScan speed： 100 nm/minResponse：2 secData interval：0.1 nmBandwidth： l nm Accumulation： 2 Measurement time： 90 sec. per sample Fig. 2 CD spectra of eight proteins Lysozyme： Cytochrome C： Concanavalin A： b-Lactoglobulin： Trypsin Inhibitor： Ribonuclease A： Human Serum Albumin： Hemoglobin： Secondary Structure Estimation In table 1， the results of secondary structure estimation of eight proteins conducted using the PLS methodon CD spectra are compared with the results of X-ray crystallography. CD-0008 File Name Creation Date Sample name Helix Sheet Turn Other 1 O：￥..￥2011 2011/10/2512：25 Lyz 42.8% 0.4% 24.4% 32.4% 2 C：￥..￥2011 2011/10/25 12：25 Cytc 42.6% 3.1% 18.1% 36.2% 3 C：￥..￥2011 2011/10/2512：25 ConA 5.1% 44.6% 13.9% 36.4% 4 C：￥.￥2011 2011/10/2512：25 S-Lactoglobulin 17.8% 35.5% 12.3% 34.4% 5 C：￥.￥2011 2011/10/25 12：26 Trypsin Inhibitor 13.9% 25.3% 17.3% 43.5% 6 O：￥￥2011 2011/10/2512：26 RNaseA 21.5% 14.7% 22.4% 41.4% 7 C：￥..￥2011 2011/10/25 12：26 HSA 66.8% 1.3% 8.2% 23.7% 8 C：￥..￥2011 2011/10/25 12：27 Hb 61.1% 0.0% 18.0% 20.9% Fig. 3 Results using multivariate SSE program Table1 Comparison with X-ray crystallography Sample name Helix (%) Sheet (%) Turn (%) Other (%) Reference Lysozyme (Lyz) PLS 42.8 0.4 24.4 32.4 X-ray 41 4 19 35 1 Cytochrome C (CytC) PLS 42.6 3.1 18.1 36.2 X-ray 42 8 9 42 1 Concanavalin A (ConA) PLS 5.1 44.6 13.9 36.4 X-ray 2 36 12 49 1 B-Lactoglobulin PLS 17.8 35.5 12.3 34.4 X-ray 13 34 13 41 1 Trypsin Inhibitor PLS 13.9 25.3 17.3 43.5 X-ray 2 33 10 55 2 Ribonuclease A (RNaseA) PLS 21.5 14.7 22.4 41.4 X-ray 22 19 11 48 1 Human Serum Albumin (HSA) PLS 66.8 1.3 8.2 23.7 X-ray 72 0 8 20 2 Hemoglobin (Hb) PLS 61.1 0 18 20.9 X-ray 75 0 10 15 1 Helix： a-helix + 310-helix， Sheet： b-strand， Turn： turns， Other： rest References (1) W. Curtis Johnson， PROTEINS： Structure， Function， and Genetics， 35， 307-312，(1999) (2) PROTEIN DATA BANK： Trypsin inhibitor：1ba7 (DSSP)， HAS： 1bm0 (DSSP) copyrightOJASCO CorporationJASCO INTERNATIONAL CO.，LTD-Sennin-cho -chome， Hachioji， Tokyo Japan IntroductionThe multivariate SSE program can be used to analyze sets of CD data. A large number of samples can beautomatically measured and efficiently analyzed using the high-throughput circular dichroism (HDX) system