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Proteins, biological polymers composed of amino acids, have primary structures indicating the
sequence of amino acids and higher three-dimensional order structures as well. The structure of
proteins defines their biological functionality and CD spectroscopy is commonly used in the study
of proteins and oligopeptide applications. Using secondary structure estimation (SSE) software, the
helix, sheet and random coil contents of unknown proteins can be quantitatively estimated from CD
spectra.
SSE software has been applied mainly in academic or research studies of protein structure.
JASCO now offers the new SSE Program based on the PLS/PCR method of multivariate analysis,
to cope with expanding usage of protein studies across a wide range of fields, su
s.cApplication NotecopyrightOJASCO CorporationJASCO INTERNATIONAL CO., LTD4-21, Sennin-cho 2-chome, Hachioji, Tokyo 193-0835, JapanTel:+81-42-666-1322. Fax:+81-42-665-6512 CD-0011 Multivariate SSE Program Introduction Proteins, biological polymers composed of amino acids, have primary structures indicating thesequence of amino acids and higher three-dimensional order structures as well. The structure ofproteins defines their biological functionality and CD spectroscopy is commonly used in the studyof proteins and oligopeptide applications. Using secondary structure estimation (SSE) software, thehelix, sheet and random coil contents of unknown proteins can be quantitatively estimated from CDspectra. SSE software has been applied mainly in academic or research studies of protein structure.JASCO now offers the new SSE Program based on the PLS/PCR method of multivariate analysis,to cope with expanding usage of protein studies across a wide range of fields, such as bio-pharmaceutical. Keywords: Protein, Secondary structure estimation, PLS, PCR Features 1. Versatile PLS and PCR multivariate analysis applied to SSE Enhanced accuracy and robustness Easy validation of calibration models F test certification GLP/GMP compliant 2. Operational flexibility Selectable reference sets Editable secondary structure fractions 3. Reference CD Spectra of 26 proteins (260-176 nm) provided as standard. Fig. 1 Cross Validation of Multivariate SSE Program Proteins, biological polymers composed of amino acids, have primary structures indicating thesequence of amino acids and higher three-dimensional order structures as well. The structure ofproteins defines their biological functionality and CD spectroscopy is commonly used in the studyof proteins and oligopeptide applications. Using secondary structure estimation (SSE) software, thehelix, sheet and random coil contents of unknown protei
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