A UHPLC method development system for efficient scouting of chromatographic elution parameters

The speed of analysis in HPLC has dramatically improved over the last decade due to the development of columns with sub-2 µm particles and the respective UHPLC instrumentation. (U)HPLC method development is, however, still a bottleneck in laboratory workflows and can take from weeks to months, especially if extensive column and eluent scouting is required to complete the development. This often limits the laboratory productivity and increases operational costs. A high degree of automation in column and eluent scouting is required to fully exploit the speed potential of UHPLC.