近红外荧光粉测量

近红外荧光标记由于其广泛的生物学应用，目前正吸引着科学界的注意。这些标签是生物研究的理想选择，因为生物分子在近红外区域吸收非常少。近红外荧光标记物在体内没有显示出不良影响，并且是高度稳定的。本应用说明使用FP-8700来获得NIR光谱区域的荧光测量值。这些数据被用来表征近红外荧光粉，以后可用作生物学研究中的荧光标记。Application NoteFP-O019 Near-Infrared PhosphorMeasurement2/3Application Note Near-Infrared Phosphor Measuremen t Introdu c tio n Nea r -inf r ared f lu orescence labe l s are cur r e n tly attracting the a t tent i on of the sc i entific commu n ity due to thei r wide r ange of biolog i cal applications. These labe l s are i deal f or biological studies since biomolecules absorb very litt l e in t h e n ea r -i n frared region. NIR fluoresce n ce l abe l s have shown no adverse effects in vivo and are h ighly stable. This application note illustrates how the FP-8700 is used to obtain fluorescence spectra and quantum y i elds of phosphors in the near-i n frared spectral r eg i on. FP-8700S p ec tro fl u oro m ete r Keyword s FP-8700， ESC-842 Calibrated WI li ght source， ILF-890 Integrating sphere， FPA-810 Powder measu r ement block， PSH-101Powder cell， Fluorescence， Nea r -i nfrared， Quantum yield E xperimental Measurement Conditions Excitation Fluorescence Emission Wavelength 883 nm Excitation Wavelength 600nm Emission Bandwidth 10nm Excitation Bandwidth 10 nm Excitation Bandwidth 10nm Emission Bandwidth 10 nm Scan Speed 500 nm/min Scan Speed 500 nm/min Response Time 0.5 sec Response Time 0.5 sec Data Interval 0.2nm Data Interval 0.2nm Sensitivity 600V Sensitivity 600V Re su l ts The e xc i tation spec t ra of YVO ：Nd3+a n d YVO：Nd 3， Yb3 are s h own in F i gure 1 a n d th e peaks are l abel e d wi t h t he i r correspond in g excited state t ra n sitions fr om the ground state of Nd3(4192) Figure 1. Excitation spectra o f YVO ：Nd +(b l u e) and YVO ：Nd3，Yb3+(red ). Th e f luor e sc e nc e spec tr a of YVO：Nd3+ a n d YVO ：Nd3+，Yb3are s h own in Figur e 2 and th e p eaks ar e lab e l e d with th e ir corr e spondi n g t ransi t io n stat e s from t h e e xci te d states of Nd3 (4F 2)and Y b 3+(2F 52) Figure 2. Fluoresce n ce spectra of YVO ：Nd*(blue) and YVO：Nd ， Yb"(r ed). 28600 Mary's Cour t ， E a s to n ， M D 21601US A Appl i c a t i on Note The quantum y i e l ds of both YVO ：Nd +and YVO：Nd3， Yb3 were ca l culated and shown in Table 1. Table 1. Quantum y i e l d res u lts . Absorbance (%) External Quantum Yield Internal Quantum Yield YVO：Nd3 9.0 2.5 28.5 YVO：Nd3，Yb3 21.0 12.4 59.1 Conclusion This application n ote uses a FP-8700 to obtain f luo r escence measurements in t h e NIR spec t ral region. T his d a ta is us e d to c h a r act e rize near-i nfrar e d phosphors， wh i c h can lat e r be u s ed as fl uor e sce n t labe l s in biological stud i es. 28600 Mary's Cour t ， E a s to n ， M D 21601US A