生物表面活性剂中水分含量检测方案(卡氏水分测定)

Weekly Application Note Category Karl Fischer Volumetric Titrator AQV series Pharmaceuticals & Cosmetics Water content in biosurfactant by Karl Fischer Volumetric Titration Key words；biosurfactant， volumetric Karl Fisher titration Outline Biosurfactants are surface-active substances， derived from biological materials， having thegreat performance as surfactant yet biodegradable and environmentally-friendly. The watercontent is the biosurfactants can be easily determined by Karl Fischer Volumetric Titrator AQVseries. Reagents Titrant HydranalQ Composite 5 Solvent HydranalQ Methanol dry or common anhydrous methanol ※HydranalQ KF reagents are available from Sigma-Aldrich www.sigma-aldrich.com/hydranal Instruments KF Titrator AQV-2200S (High-end， Color LCD touch screen)AQV-300 (Simple & Compact) Simple and compact! ANEW AQV-2200S (built-in printer， touch panel) AQUA COUNTER Procedure ①Fill the titration cell with dry methanol and remove the water containedin the cell by performing a blank titration. Wait until the potential mVbecomes stable. ②Weigh the total weight of the container involving the sample， funnel， andspatula [S1(g)]. ③Press SAMPLE key，remove the common stopper on the cell lid， attachthe funnel and introduce about 0.2g of the sample with the spatula throughthe funnel.And attach the stopper immediately to close the cell. ④FPress TITRATION key to start a titration. Set “Interval Time” to 30 sec.to determine the end point. ⑤ Weigh the sample container， funnel and spatula accurately [S2(g)] to obtain the amountof the sample injected by subtracting S2 from S1. And then enter the value. Condition parameters Factordetermination Measurement Cal Mode 7 0 Interval Time 30 sec 30 sec S-Timer 0 min 0 min T-Timer 0 min 0 min Buret Speed (Out) 5 mL/min 5 mL/min Buret Speed (IN) 12mL/min 12 mL/min Blank Value 0mL 0 mL Cal Factor TIT Buret No. ON 1 Buret 1 Factor Result of factor Unit Mode --- AUTO Min. Feed Vol. 0.01 mL 0.01 mL Max Volume 20 mL 20 mL CP level 200 mV 200 mV End Mode 1 1 Auto Interval 0 0 本实验采用容量法对生物表面活性剂进行水分含量测试。