赛默飞LTQ XL线性离子阱质谱仪

双压线性离子阱质谱用于单克隆抗体分子量测定

在本文的研究中，我们使用Velos Pro 双压线性离子阱对完整单克隆抗体及其轻重链进行了分子量测定。结果表明：首先，对于完整单克隆抗体，离子阱质谱可以实现分子量测定并分辨出不同糖型；对于经DTT 还原后的轻、重链，离子阱质谱同样可以测定其分子量和不同糖型。离子阱质谱可满足传统定性分析需求，是未知化合物结构解析的强大武器；在蛋白质药物研究方面，被广泛用于肽图分析、序列鉴定和翻译后修饰研究等。通过本文的研究，我们证明了离子阱质谱同样可以用于单克隆抗体分子量的测定。同时，由于离子阱质谱可对化合物进行多级质谱解析，在肽段的翻译后修饰研究、尤其是糖链的精细结构解析中起到不可或缺的作用。另外，双压线性离子阱Velos Pro同时具有高压和低压阱，高压阱提供最大化离子捕获、裂解和隔离效率，低压阱提供其他离子阱质谱所无法比拟的分辨率和扫描速率。综上，离子阱质谱亦为生物制 药行业相关分析的有力工具。

LTQ鉴定和研究人肥厚型心肌病的假定和已知血浆生物标记物及其差异表达

This workflow involved the analysis of a highly complex protein digest and was dependent upon an LC/MS methodology that achieves the best possible peptide detection and analysis, in order to detect important, low-level protein components. In addition to this, many of the peaks used for quantification were small, but accurate comparisons were possible due to the large number of data points defining these peaks. The LTQ acquires five MS or MS/MS spectra/second, ensuring the best possible data for accurate analytical determinations such as these. Up-Regulation of CRP, as measured by a 2.2-fold increase in its raw MS signal (concentration) at T24 vs T0, is consistent with the observed general elevation of acutephase response factors of which it is a part. Future work will involve validation of unique putative markers that were also identified in this analysis.