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当前位置: 安捷伦 > 解决方案 > ELSD触发制备的收集参数优化

ELSD触发制备的收集参数优化

2018/09/03 14:37

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应用领域:
制药/生物制药
发布时间:
2018/09/03
检测样品:
其他
检测项目:
其他
浏览次数:
109
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方案摘要:

On the Agilent OpenLAB CDS, fraction collection can be performed with a UV detector, or any additional detector, such as an Evaporative Light Scattering Detector (ELSD). In another Technical Overview1 , we described a simple way to split the preparative fl ow on the Agilent preparative LC systems to trigger the collection with an ELSD. This Technical Overview shows how to optimize peak-based fraction collection triggered by an ELSD. We describe the system setup, the delay calibration between detector and fraction collector, and the use of the variable light sensitivity of the Agilent 1260 Infi nity Evaporative Light Scattering Detector. We also discuss the peak-based fraction collection settings required to achieve the best purity and recovery.

产品配置单:

分析仪器

Agilent 1260 Infinity II 制备型液相色谱

型号: 1260 Infinity II Preparative

产地: 德国

品牌: 安捷伦

面议

参考报价

联系电话

分析软件

OpenLAB 软件

型号: OpenLAB

产地: 美国

品牌: 安捷伦

面议

参考报价

联系电话

Agilent 1290 Infinity II Multisampler

型号: 1290 Infinity II Multisampler

产地: 德国

品牌: 安捷伦

面议

参考报价

联系电话

方案详情:

Abstract 

On the Agilent OpenLAB CDS, fraction collection can be performed with a UV detector, or any additional detector, such as an Evaporative Light Scattering Detector (ELSD). In another Technical Overview1 , we described a simple way to split the preparative fl ow on the Agilent preparative LC systems to trigger the collection with an ELSD. 

This Technical Overview shows how to optimize peak-based fraction collection triggered by an ELSD. We describe the system setup, the delay calibration between detector and fraction collector, and the use of the variable light sensitivity of the Agilent 1260 Infi nity Evaporative Light Scattering Detector. We also discuss the peak-based fraction collection settings required to achieve the best purity and recovery.

Introduction

The evaporative light scattering detector (ELSD) is commonly used to detect compounds without a chromophore. Therefore, it is ideal for the purifi cation of amino acids, peptides, carbohydrates, and lipids. Using the Agilent Splitter Kits corresponding to the required purifi cation scale on the Agilent Purifi cation System1 , the signal from the ELSD can be used for peak-based collection of the target compounds. To ensure the best reliability and purity of the collected fractions, several parameters need to be optimized. 

The delay time between the detector and the fraction collector needs to be measured. This delay is the difference between the time the compound is monitored in the detector, and the time when this compound reaches the diverting valve of the fraction collector. Correct measurement of this delay time is an important step of the system optimization. This procedure is automated on the Agilent 1260 Infinity Fraction Collectors using Agilent Lab Advisor Software. 

This Technical Overview also describes how to improve parameters such as the light intensity of the ELSD, and the fraction collector settings.

Conclusion 

This Technical Overview shows how to optimize fraction collection with the Agilent 1260 Infi nity Evaporative Light Scattering Detector. The delay calibration performed with the Lab Advisor Software provides a simple and quick method to calibrate the delay volume between detector and fraction collection. With the possibility of light intensity adjustment, the 1260 Infi nity ELSD offers a fl exible solution for preparative liquid chromatography by increasing or decreasing the sensitivity of the detector with respect to the sample concentration. These features ensure best reliability of the purifi cation results.

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Agilent 1260 Infinity II 制备型液相色谱

型号:1260 Infinity II Preparative

面议

OpenLAB 软件

型号:OpenLAB

面议

Agilent 1290 Infinity II Multisampler

型号:1290 Infinity II Multisampler

面议

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