Agilent 6475 三重四极杆液质联用仪

Triple quadrupole LC/MS systems have become widely accepted as a platform for targeted, large-batch, sample analysis on a day-to-day basis. A primary concern for routine/targeted analysis is instrument stability; which can vary over time due to the soiling of crucial ion optics components. While incorporating an internal standard and measuring abundance ratio may help alleviate signal drift from a data-analysis standpoint, it does not give key indications to the quality of the instrument’s health.

Large output production labs often resist changes in physical hardware or software revision despite improved performance or enhanced features due to the time consuming nature of re-verification, re-validation, or reconstruction of the analytical method. Often, the method needs to be “rebuilt”, using the former method as a template – possibly leading to transcription errors or unexpected performance differences.

Developing Multiple Reaction Monitoring (MRM)-based screening methods for routine analysis is a common operation in analytical laboratories. To maximize analytical performance, researchers often need to optimize many LC and MS parameters. An intelligent software could assist method development from several aspects, such as cutting method development time, automating workflow, auto-selecting optimal parameters, tracking system to meet compliance requirement, etc.

摘要 亚硝胺杂质是药品生产过程中产生的痕量副产物。这些杂质被归类为潜在遗传毒性杂质，长期摄入可能致癌。因此，以高灵敏度和可靠性测定它们在成品药中的含量水平非常重要。本应用简报评估了 Agilent 6475 三重四极杆 LC/MS (LC/TQ) 系统、Agilent 1290 Infinity II 生物液相色谱系统和大气压化学电离 (APCI) 离子源组合对8 种亚硝胺杂质的定量性能。

Agilent 6475 三重四极杆液质联用系统

There is a global trend towards more self‑aware, intelligent tools meant to make life easier. The field of liquid chromatography/mass spectrometry is no different with the move to automate difficult and challenging tasks, making mass spectrometry more accessible. These advances increase instrument uptime, streamline workflows, save time with immediate validated results and ultimately reduce cost-of-ownership.

亚硝胺杂质是药品生产过程中产生的痕量副产物。这些杂质被归类为潜在遗传毒性 杂质，长期摄入可能致癌。因此，以高灵敏度和可靠性测定它们在成品药中的含量 水平非常重要。本应用简报评估了 Agilent 6475 三重四极杆 LC/MS (LC/TQ) 系统、 Agilent 1290 Infinity II 生物液相色谱系统和大气压化学电离 (APCI) 离子源组合对 8 种亚硝胺杂质的定量性能。

Agilent 6475 三重四极杆液质联用系统立足于安捷伦深受客户信赖的 LC/TQ 平台，代表了下一步的仪器发展方向。新加入的功能创新为您的实验室赋能，满足不断增长的样品 通量与分析效率要求。

Pesticide residues remaining in or on commodities raise concerns for food safety and environmental impact. Regulatory agencies have published policy statements to guide agricultural organizations on the proper use of pesticides and established Maximum Residue Limits (MRLs) of pesticides in various food matrices. Thus, there are widely demands for highly sensitive and robust platform for the quantification of pesticide residues analysis..

The act of developing targeted triple quadrupole LC/MS (LC/TQ) methods from start to finish is a complex and time-consuming, multi-step workflow. Method development becomes even more challenging if former mass spectrometry parameters for each analyte were not established – especially in the case of novel compounds. In such cases, “by-hand” optimization and characterization is needed to obtain the most effective MRM parameters.

This application note describes the development and valuation of a comprehensive LC/MS/MS method for over 500 pesticide analyses in three food matrices (wheat, olive oil, and black tea). The work was completed using the Agilent 6475 triple quadrupole LC/MS (LC/TQ) system coupled with the Agilent 1290 Infinity II Bio LC system and MassHunter Workstation 12.0.

Large output production labs often resist changes in physical hardware or software revision despite improved performance or enhanced features due to the time consuming nature of re-verification, re-validation, or reconstruction of the analytical method. Often, the method needs to be “rebuilt”, using the former method as a template – possibly leading to transcription errors or unexpected performance differences.