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TBE-20A分析型高速逆流色谱仪/萃取仪/制备色谱仪

TBE-20A分析型高速逆流色谱仪/萃取仪/制备色谱仪

TBE-20A分析型高速逆流色谱仪/萃取仪/制备色谱仪

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9.5 分

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同田

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分析型高速逆流色谱仪

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--

产地

中国大陆

上海同田生物技术有限公司-高速逆流色谱仪HSCCC

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第18年
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产地类别： 国产

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仪器简介：

• 三分离柱专利：在高速运转时保持最佳平衡，可以使溶剂分离体系达到较高的保留率，同时，有效保证设备稳定性，使运行噪音更小。
• 前处理工序简单，简化提纯步骤，节约提纯时间。
• 高分离率：能把天然产物有效成分分离提纯到 99% 以上。
• 高回收率：不存在因固态载体造成的吸附损耗、样品变性等问题，理论上样品回收率接近100%。
• 使用成本降低：不用柱填料，不用更换分离柱，仪器的运行和维护成本低。
• 附加温控功能：实现精确控温。精确控制分离过程的温度，有助于摸索实验条件，提高实验重复性；样品分离不受环境温度的影响，可通过调节温度改善样品的分离度，拓宽溶剂体系的选择范围。

技术参数：

分离量：微克-毫克级

主机容量： 20ml　　　　　　　　　　进样体积：20ul(随机供应)

进样圈：5ul-2ml(选购)                                进样阀：Rheodyne 7725i

转速范围：0-2000 转/分　　                    分离转速：1400-2000 转/分（无级变频调速）　

流速范围：0.1-5.0ml/min　　　　         分离流速： 0.5-1.5ml/min

推荐工作转速：1800 转/分                        推荐工作流速：1.0ml/min

压力：0-2MPa

紫外检测器波长：254、280nm（另可选择全波长检测器）

温控模块（接循环水浴）：温度调控范围15～40℃，精度0.5℃，温控循环液量 1～10 L/min

额定电压·功率：220V±10%，50/60Hz·300W

主机尺寸：330×600×550mm

主要特点：

三分离柱设计，双六通阀设计，提供在线检测；

分离时间短，效率高，溶剂消耗小，适合进行前期最佳分离工艺的研究、指纹图谱的研究和样品分析检测研究。

采用HPLC 7725i样品进样阀

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仙茅中仙茅甙和仙茅甙乙的分离检测方案(高速逆流色谱)
Abstract: A preparative high-speed counter-current chromatography (HSCCC) was used to isolate and separate curculigoside and curculigoside B from the famous traditional Chinese medicinal herb Curculigo orchioides. Some parameters including two-phase solvent system, separation temperature,flow rate of the mobile phase and revolution speed of the apparatus were all investigated, and a successfully isolation and purification was achieved at the following conditions: the two-phase solvent system composed of ethyl acetate-ethanol-water at a volume ratio of 5:1:5(v/v/v) was selected and the lower phase of the system was used as the mobile phase at the flow rate of 2.0ml/min, and the isolation temperature and revolution speed were set at 30◦C and 800rpm.The isolation produced a total of 14.5 mg curculigoside B and 72.8 mg curculigoside with purities of 96.5% and 99.4% determined by high performance liquid chromatography (HPLC) from 300 mg crude extract after cleaning-up by D101 macroporous resin,which was necessary for the excellent purification. The recoveries of the two compounds were 91.6% and 92.5%, respectively, and the chemical structure identification was carried out by UV, MS and NMR. This separation method was more effective than some conventional techniques.
制药/生物制药 2017-09-21
南沙参根茎中化学成分检测方案(高速逆流色谱)
Abstract: Preparative high-speed counter-current chromatography (HSCCC), as a continuous liquid–liquid partition chromatography with no solid support matrix, combined with evaporative light scattering detection (ELSD) was employed for systematic separation and purification of non-chromophoric chemical components from Chinese medicinal herb Adenophora tetraphlla (Thunb.), Fisch. Nine compounds, includingα-spinasterol, β-sitosterol, nonacosan-10-ol, 24-methylene cycloartanol, lupenone, 3-O-palmitoyl-β-sitosterol, 3-O-β-d-glucose-β-sitosterol, eicosanoic acid and an unknown compound, were obtained. The compounds were all above 95% determined by high-performance liquid chromatography (HPLC)–ELSD, and their structures were identified by H-NMR and chemical ionization mass spectroscopy (CI-MS). The results demonstrate that HSCCC coupled with ELSD is a feasible and efficient technique for systematic isolation of non-chromophoric components from traditional medicinal herbs.
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木蝴蝶中黄芩素和白杨黄素检测方案(高速逆流色谱)
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Abstract: A preparative high-speed counter-current chromatography (HSCCC) was used to isolate and separate curculigoside and curculigoside B from the famous traditional Chinese medicinal herb Curculigo orchioides. Some parameters including two-phase solvent system, separation temperature,flow rate of the mobile phase and revolution speed of the apparatus were all investigated, and a successfully isolation and purification was achieved at the following conditions: the two-phase solvent system composed of ethyl acetate-ethanol-water at a volume ratio of 5:1:5(v/v/v) was selected and the lower phase of the system was used as the mobile phase at the flow rate of 2.0ml/min, and the isolation temperature and revolution speed were set at 30◦C and 800rpm.The isolation produced a total of 14.5 mg curculigoside B and 72.8 mg curculigoside with purities of 96.5% and 99.4% determined by high performance liquid chromatography (HPLC) from 300 mg crude extract after cleaning-up by D101 macroporous resin,which was necessary for the excellent purification. The recoveries of the two compounds were 91.6% and 92.5%, respectively, and the chemical structure identification was carried out by UV, MS and NMR. This separation method was more effective than some conventional techniques.
制药/生物制药 2017-09-21
南沙参根茎中化学成分检测方案(高速逆流色谱)
Abstract: Preparative high-speed counter-current chromatography (HSCCC), as a continuous liquid–liquid partition chromatography with no solid support matrix, combined with evaporative light scattering detection (ELSD) was employed for systematic separation and purification of non-chromophoric chemical components from Chinese medicinal herb Adenophora tetraphlla (Thunb.), Fisch. Nine compounds, includingα-spinasterol, β-sitosterol, nonacosan-10-ol, 24-methylene cycloartanol, lupenone, 3-O-palmitoyl-β-sitosterol, 3-O-β-d-glucose-β-sitosterol, eicosanoic acid and an unknown compound, were obtained. The compounds were all above 95% determined by high-performance liquid chromatography (HPLC)–ELSD, and their structures were identified by H-NMR and chemical ionization mass spectroscopy (CI-MS). The results demonstrate that HSCCC coupled with ELSD is a feasible and efficient technique for systematic isolation of non-chromophoric components from traditional medicinal herbs.
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本实验结果表明采用高速逆流色谱法可以从淡豆豉中分离得到质量分数的大豆素和染料木素单体化合物，分离时间较短，操作简便、样品无损失。由此可以进一步制备获得这两个物质的化学对照品，为淡豆豉深入研究和质量控制提供了必要的物质基础。
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摘要：建立了高速逆流色谱分离纯化木蝴蝶黄芩素和白杨黄素的方法。两相溶剂系统为石油醚2乙酸乙酯2甲醇2水,固定相为5∶5∶5∶5(V/V)体系的上相,以5∶5∶5∶5(V/V)和5∶5∶7∶3(V/V)体系的下相为流动相进行梯度洗脱。从300mg木蝴蝶粗提物中一步分离纯化得到2515 mg黄芩素和3616 mg白杨黄素。经高效液相色谱分析,纯度分别为9912%和100%。其化学结构由1H2NMR 和13C2NMR鉴定。
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相关仪器相关资料

高速逆流色谱应用研究

摘要本文介绍了高速逆流色谱技术的工作原理、特点，综述了近年来高速逆流色谱(HSCCC)在分离制备天然产物、蛋白质、抗生素、无机物等领域的进展，尤其是近几年在中药尤其是植物药有效成分分离纯化方面的应用情况，总结了适用于HSCCC的溶剂体系，并展望了HSCCC与质谱、蒸发光散射联用等新技术的应用前景。

141MB 2008-11-07

用户评论

综合评分：9.5分

发评论

该高速逆流色谱仪的萃取功能十分强大。通过优化的样品前处理步骤，它能够从复杂的样品矩阵中提取目标物质，消除干扰，提高检测灵敏度。

2023-07-03 13:09:43

问商家

同田高速逆流色谱分析型高速逆流色谱仪的工作原理介绍

高速逆流色谱分析型高速逆流色谱仪的使用方法?

同田分析型高速逆流色谱仪多少钱一台?

高速逆流色谱分析型高速逆流色谱仪可以检测什么？

高速逆流色谱分析型高速逆流色谱仪使用的注意事项？

同田分析型高速逆流色谱仪的说明书有吗？

同田高速逆流色谱分析型高速逆流色谱仪的操作规程有吗？

同田高速逆流色谱分析型高速逆流色谱仪报价含票含运吗？

同田分析型高速逆流色谱仪有现货吗？

TBE-20A分析型高速逆流色谱仪/萃取仪/制备色谱仪信息由上海同田生物技术有限公司-高速逆流色谱仪HSCCC为您提供，如您想了解更多关于TBE-20A分析型高速逆流色谱仪/萃取仪/制备色谱仪报价、型号、参数等信息，欢迎来电或留言咨询。

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