HORIBA OpenPlex表面等离子体共振成像仪

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HORIBA

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型号

OpenPlex

荣誉奖项

产地

欧洲

HORIBA（中国）

白金
第21年
生产商

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产地类别： 进口

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仪器简介：

HORIBA scientific将等离子体共振技术、成像技术和微阵列芯片技术进行结合，可以一次获取百种生物分子相互作用的信息，这种这列的测量方式突破了传统通道式测量的局限，特别适用于快筛及实时成像的应用需求。适用于各类小分子化合物、多肽、蛋白质、寡核苷酸和寡聚糖直至类脂、噬菌体、病毒和细胞等生物体系的免标记分析，广泛用于蛋白质组学研究，癌症研究，新药研发，信号传递，多分子复合物的结构和组装，分子识别，免疫调节，免疫测定法，疫苗开发，瞬时结合，配体垂钓，结合特异性，结构与功能的关系，酶反应等应用领域。

SPRi-OpenPlex灵活式表面等离子体共振成像系统（生物分子相互作用仪）是一款免标记、多通道生物分析和研究的理想工具。该系统设计精巧，可实时监测相互作用并获得动力学参数。开放式结构设计，适用于多种应用需求。

可选附件：

l 蠕动泵

l 注入系统

l 自动脱气装置

l SPRi-Array快速台式点样仪

l SPRi-Biochips™生物芯片（CS/CO/CSe/COe/CTg/CH功能化）

技术参数：

l 样品体积：200μL（标配），100/250/500μL等可选

l 样品浓度：300ng/mL(100~1000KDa)~10μg/mL(4~20kDa)

l 样品分子质量>240Da

l 检测下限：10pg/mm²

主要特点：

l 阵列式检测，实时成像，直观显示反应过程

l 蛋白质、多肽、DNA和细胞等免标记分析

l 动力学曲线实时监测

l 直接扣除阴性对照

l 外置部件选择性灵活

注：具体配置、价格请咨询当地销售工程师

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Results demonstrate that monolayer surfaces permit accurate SPR analysis of macromolecular interactions and that the SPRi-PLEX configuration is well suited to a simple recovery protocol that provides rapid sensitive mass spectrometry analysis. Clearly at these levels of surface saturation more than 100 spots could be rapidly visualized and the material recovered for analysis by SELDI even with the manual approach used here.
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Results demonstrate that monolayer surfaces permit accurate SPR analysis of macromolecular interactions and that the SPRi-PLEX configuration is well suited to a simple recovery protocol that provides rapid sensitive mass spectrometry analysis. Clearly at these levels of surface saturation more than 100 spots could be rapidly visualized and the material recovered for analysis by SELDI even with the manual approach used here.
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The GenOptics platform, centred on SPRi technology, enables: - The generation of biochips bearing a large range of peptide probes (peptide arrays) - The label-free detection of peptide-antibody interactions in complex media such as undiluted sera varied according to the patient and the peptide, revealing donor-related differences in the anti-HCV antibody responses (specificity, amount and affinity). The GenOptics SPRi technology is a powerful tool for clinical applications such as diagnosis, pharmacologic studies, and for research.
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We demonstrate here the robustness of our system. We performed 112 ovalbumin injections each followed by injection of regeneration buffer (Glycine/HCl). After more than one hundred regenerations, the biochip remains active as indicated on histogram.
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Combining Polypyrrole with SPRi provides a unique method for fast, easy and reliable studies of oligosaccharide interactions on a biochip. The GenOptics platform, based on SPRi technology, is a powerful device for glycobiology, a rapidly expending area of research. This study demonstrates the process of grafting natural oligosaccharides on SPRi-Biochip™. SPR imaging is ideal to study protein-oligosaccharide interactions without labeling neither the probes nor the targets and to monitor multiple (at least 25) kinetic interactions in real time. This approach enable direct studies of synthetic or natural oligosaccharides properties to be developed even further.
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