HORIBA OpenPlex表面等离子体共振成像仪

The GenOptics platform, centred on SPRi technology, enables: - The generation of biochips bearing a large range of peptide probes (peptide arrays) - The label-free detection of peptide-antibody interactions in complex media such as undiluted sera varied according to the patient and the peptide, revealing donor-related differences in the anti-HCV antibody responses (specificity, amount and affinity). The GenOptics SPRi technology is a powerful tool for clinical applications such as diagnosis, pharmacologic studies, and for research.

Using GenOptics SPRi sensitive technology (Surface Plasmon Resonance imaging) a method was developed to detect DNA point mutations.

GenOptics SPRi technology allows a real time study of biomolecular interactions. No additional labelling reagent is required, and a large number of spots can be analysed at the same time and in the same condition. This technique is therefore proven to be efficient at performing accurate high throughput antibody screening. ?

Combining Polypyrrole with SPRi provides a unique method for fast, easy and reliable studies of oligosaccharide interactions on a biochip. The GenOptics platform, based on SPRi technology, is a powerful device for glycobiology, a rapidly expending area of research. This study demonstrates the process of grafting natural oligosaccharides on SPRi-Biochip™. SPR imaging is ideal to study protein-oligosaccharide interactions without labeling neither the probes nor the targets and to monitor multiple (at least 25) kinetic interactions in real time. This approach enable direct studies of synthetic or natural oligosaccharides properties to be developed even further.

We demonstrate here the robustness of our system. We performed 112 ovalbumin injections each followed by injection of regeneration buffer (Glycine/HCl). After more than one hundred regenerations, the biochip remains active as indicated on histogram.

We demonstrate here that the SPRi technology developed by Genoptics is thoroughly suited to detect small molecules, such as biotin, with molecular weights below 500 Da. Biotin captured on pyrrole-streptavidine spots and electro-grafted on SPRi-Biochip is specific and shows the high sensitivity of our SPRi technology instruments.