HORIBA XploRA INV多功能拉曼及成像光谱仪

The flexibility provided by the inverted microscope configuration of the LabRAM INV is ideally suited to in situ Raman measurements within microreactor channels. Information on flow properties, mixing and reactions within channel networks can be obtained, allowing reactions to be fully optimised. In particular, the Raman concentration imaging method described in this application note provides a valuable tool for testing and validating mathematical models of organic reactions in these microchannel systems. reactions within channel networks can be obtained, allowing reactions to be fully optimized, In particular, the Raman concentration imaging method described in this application note provides a valuable tool for testing and validating mathematical models of organic reactions in these microchannel systems.

把激光拉曼光谱仪和FT-IR红外拉曼光谱仪有机地整合 在同一系统中，这样可以获得完全的谱信息，还减小 了仪器的体积、节约经费。特别需要指出的是，由于 该系统采用了“共点”技术，它可以保证使我们在同 一样品的同一位置上获取两种谱信息（不同的光谱范 围）。这完全不会影响两种仪器（激光拉曼光谱仪和F T-IR红外拉曼光谱仪）各自的性能和技术特点。该系 统，在众多研究领域（例如：聚合物性质、缺陷特 性、病理学、法医学、药物学、绘画颜料等）中都有 十分有意义的应用价值。

拉曼光谱已经成为石墨烯的表征和改性研究中必不可少的工具，在石墨烯的层数、缺陷表征，以及掺杂、堆叠、层间嵌入、环境影响等方面的研究起着非常重要的作用。拉曼成像不仅可直观的表征不同层石墨烯层数的空间分布，还可以表征石墨烯掺杂、形变引起的拉曼峰位、峰形变化的分布。

通过将拉曼光谱与湿度控制单元联合使用，为准确的跟踪在一定环境条件（湿度、温度）下药物的结构变化提供了可能。这种结合不仅能够用于检测样品的水化（如本案例中的乳糖及茶碱），也可以用来表征多晶型或相变，由水分引起的膨胀或退化。该湿度控制单元能够与拉曼成像联用，可以用来分析异构样品—如片剂药品结构的变化。

一般情况下利用拉曼光谱技术可以非常方便的鉴定物质成分，获得结构信息。但是，一些化学物质直接通过拉曼光谱无法检测出信号，需要通过拉曼增强技术，提高拉曼信号信噪比，从而检测出待检物质。

Raman microscopy has been used to interrogate single bacteria cells – with multivariate analysis it has been shown that different species and growth phases can be quickly distinguished. Cells grown with differing amounts of 13C enriched nutrients showed Raman bands shifted to lower wavenumber due to the heavy isotope. With further multivariate analysis the cells can be classified according to the percentage uptake of 13C.

It has been shown that the analyte rhodamine 6G can be successfully measured and identified within single living cells using surface enhanced Raman microscopy. Principal components analysis for covariant features in the spectra allowed the identification of rhodamine 6G despite extensive spectral overlap from the cellular matrix.

本文列举了Horiba Scientific及其拉曼用户在生命科学研究（包括基础研究、生物医学、药物、化妆品以及食品）中的一些应用实例，显示了共聚焦拉曼技术、新的拉曼成像方法可为该领域的应用提供坚实的技术支持。

The phenomenon of diffusion has been known for centuries. For example,the ancients who dyed fabrics certainly noticed the leading edge of their turquoise dye propagating through their cotton cloth, and biologists have long been aware of osmosis through various membranes. With our century’s advances in engineered materials, scientists and engineers are now able to design diffusive layers to provide controlled rates and selectivity of diffusion. There are a number of applications of engineered diffusion in current technology. Some are medical: drug patches allowing sustained slow release of a pharmaceutical, and membranes are used in dialysis and various laboratory separation procedures. Similar to the use of drug patches is the agricultural use of slow-release nodules to provide sustained fertilisation of soil. Industrial production of chemicals and foodstuffs often depend on separations with various membranes. Other applications are of a less organic nature, as in the separation of gases by selective diffusion through membranes, and of ions through a solid, which might be part of a battery or other electronic device. Sometimes, diffusion is an undesirable phenomenon, as when we wish to store gases dissolved in a solid, and they can diffuse out over time, or when diffusion of oxygen into a semiconductor crystal can depreciate the performance of an electronic device.

显微共焦拉曼光谱仪优异的空间分辨率不仅可用于聚合物的单点检测，还可用于平面及纵向的成像扫描，获得空间分布及纵向各层分布及厚度。此外，还可远程在线监控聚合物的反应过程，对其进行实时测定。

拉曼光谱可与AFM联用，不仅可获得聚合物薄膜的表面形貌图，还可以获得拉曼光谱及拉曼成像图，可多元化的信息共同全面的表针此聚合物。

拉曼光谱在脂肪酸探测中具有为重要的应用，可以表征动物和植物油的来源。本文使用散射和透射拉曼，结合多种分析方法，包括单点分析，XY成像以及多变量分析，对脂肪酸进行系统的分析。

拉曼光谱对生物分子的细微变化非常敏感，本文使用拉曼光谱对小麦的谷物进行成像分析，获得了谷物中淀粉、脂质及蛋白质的分布；并通过对一系列硬度不同的谷物进行分析，了解了蛋白质结构与谷物硬度间的关系。

Thanks to confocal Raman spectroscopy we have been able to investigate in vivo the epidermis chemical composition in its thickness. In depth measurements allowed us to observe modifications of the relative concentration of some components (water, proteins) depending on the depth in the skin and on its state of hydration. The use of larger numerical aperture objectives would be helpful to emphasize the in-depth variations because of the possible gain in axial resolution and therefore in-depth discrimination. Indeed, the operator could then probe in depth with a smaller step size. As a possible gain on the above measurements, the use of more powerful commercially available NIR diode lasers would yield up to ten times more laser power at the sample (60-80 mW) and would result in a tremendous improvement in terms of signal collection and integration time. ?