HORIBA Fluorolog®-3科研级荧光光谱仪

PL and PLE spectra of a GaN sample at various temperatures. ?

近日郑州大学史志锋团队，成功利用无铅钙钛矿，制备出一种紫外窄带光电探测器。它具有高的光谱选择性，不仅填补了无铅钙钛矿在紫外窄带探测器的研究空白，也为实现无铅紫外光电探测器在全波段的商业化应用，提供了新的思路和可能。

马萨诸塞大学韩刚博士就在这一方面做出了突破，他成功 制备出一种新的近红外光敏剂。 这种光敏剂能够穿透深层 组织，不仅能解决光动力学疗法穿透能力差的问题，同时 还能提高光敏剂的反应效率 （在近红外范围表现出显著增 强的吸收和单态氧生成效率）。这一突破不仅是首创性的， 且具有非常重要的意义，有望 将光动力学疗法推向深层组 织和大尺寸的癌症治疗 。

四川大学吴鹏教授团队与加拿大 Waterloo 大学刘珏文教授团队 合作，改变思路，聚焦于单线态氧产生的光物理和光化学过程， 通过镧系金属离子添加，助力提升光敏剂单线态氧产率。

Cyclodextrin-enhanced room-temperature phosphorescence (CD-RTP) shows promise as a sensitive analytical technique. Sample preparation is simple, data-acquisition and processing are easily automated with a Fluorolog® spectrometer, and the result is an intense, well-structured RTP signal. CD-RTP offers the advantages of increased selectivity based on molecular geometry, and partial immunity to quenching by dissolved O2 molecules.

For researchers of SWNTs, we offer the NanoLog® and Nanosizer®. Researchers of quantum dots can use our Fluorolog . For OLED technology, we have TCSPC instruments to resolve fluorescent lifetimes. HORIBA Jobin Yvon has the optimum spectrofluorometer for nanotechnology research in all these areas.

Because it offers sensitive fluorescence measurements, full system control with our exclusive FluorEs-sence™ software, and modular construction, the Fluorolog spectrofluorometer is ideal for kinetic fluorescence determinations. The calibration procedure described herein can be applied readily to reaction-rate determinations® with your Fluorolog system.

This technical note describes kinetic fluorescence as an analytical technique to quantify non-fluorescent species. The technique is applied to the determination of thiamine (vitamin B1) in solution. Fluorescence intensity is monitored as thiamine converts to fluorescent thiochrome, and a relationship is established between the rate of increase of intensity and the concentration of thiamine. The technique is shown to have good sensitivity and selectivity.

Fluorescence spectroscopy has demonstrated a way to characterize and quantify skin aging and photoaging. The Fluorolog® allows direct in-vivo monitoring of endogenous skin fluorescence, which makes it possible to follow markers (tryptophan and collagen) in skin, and their changes in both healthy and diseased tissue. It also provides the possibility of evaluating the effect on skin from the modifications of these native proteins or the induction of The Fluorolog® will find a wide range of applications in skin research and photo-dynamic therapy.

Polarization measurements using HORIBA Jobin Yvon spectrofluorometers are a sensitive tool for probing many biochemical interactions.

A Fluorolog® spectrofluorometer, equipped with a 450-W xenon lamp, single-grating monochromators, and near-IR accessories (DSS-IGA020L InGaAs detector and emission grating at 600 grooves mm , blaze = 1000 nm) was used to measure the 1O2 emission spectrum. A high-pass filter (λ = 780 nm) acted as an order-sorter, necessary when such large wavelength-ranges are spanned. Fig. 1 shows the emission spectrum with an integration time of 0.1 s and a bandpass of 12 nm. Note the excellent signal-to-noise ratio.

Food science can benefit by the analytical information available from fluorescence. Especially useful for agricultural and biological studies are the following FLUOROLOG® features: • effective stray-light refection • a fiber-optic probe for large and difficult samples • the unique ability to measure fluorescence spectra in the IR region of the spectrum

Biological processes can be traced through study of gene-expression via a “molecular beacon” (singlestranded DNA, ssDNA), a hairpinshaped oligonucleotide with a fluorophore (donor) and a quencher (acceptor). The hairpin’s stem has two ends of complementary DNA (cDNA) that pair up. When hybridized, the fluorophore and quencher are close, producing little or no fluorescence. Molecular beacons are used to study enzyme interactions, cDNA sequencing, and biosensing.1

Polarized light striking a fluorescent molecule results in polarized fluorescence. This polarized emission gradually returns to unpolarized fluorescence depending on rotational diffusion and other factors. Anisotropy is directly related to the polarization, and is the ratio of the polarized-light component to the total light intensity. With optional polarizers installed in a spectrofluorometer, we define light intensities: IVV is with excitation and emission polarizers mounted vertically;

电致荧光变色分子开关，在一定电压刺激下可以呈现出不同荧光变色现象，展现出不同的荧光发射。因为这一特殊性质，电致荧光变色分子开关颇具发展潜力，譬如国立台湾大学Liou课题组报道的一类非共轭的聚苯胺类电致荧光变色材料。它量子产率高（21%）、对比度大（242）、响应速度快（0.4s），是一类非常具有商业价值的材料。

The Fluorolog is a sensitive instrument for all fluorescence applications in the liquid or solid state. With its ability to add detectors easily, the full range of fluorescence—from UV to near-IR—is available for all your fluorescence needs.

HORIBA Scientific提供光纤适配器和针孔适配器等附件，实现显微镜与荧光光谱仪（Fluorolog-3和FluoroMax-4）的耦联，获得高空间分辨率的荧光量子点成像，织物的矩阵扫描，微球成像以及宽视场荧光成像等显微荧光分析。

富含蛋白质的作物是所有国家的主要饮食。提高农作物的产量对于任何国家人民生活的幸福指数是很重要的，因为人们只有在食物充足的情况下才能进行其他更加高级的人类活动。无法控制的天气变化，如降雨量、霜冻灾害、风蚀等因素都会影响农作物的收成。通过使用灌溉、施肥等措施可以缓解或至少部分抵消上述天气变化带来的损失。为了采取合适的措施来抵消这些外界因素的影响，农民必须根据农作物生长时处于不同时期采取不同的对策来提高农作物产量。而这些信息可以从分析植物的荧光中得到。

根据国家卫生研究所2018年公布的一项研究，对于无法手术的癌症类型，光动力学疗法有着非常广阔的应用前景，如从骨骼开始病变的骨肉瘤就是典型。

The FLUOROLOG® system provides a quantitative assessment of tooth decay, via the FLUOROLOG®’s exclusive strong stray-light rejection, and ability to detect IR fluorescence. The FLUOROLOG®-Tau accessory provides an additional dimension of discrimination. With these data, new dental treatments and products may be assessed. Further work may lead to a quick, convenient method of tracing decay.

研究人员先采用低温溶液法，制备出无铅金属卤化物 Cs3Cu2I5薄膜，发现该无铅钙钛矿膜具有良好的结晶质量， 对紫外光也有良好的吸收 。

史教授团队将CsCu2I3薄膜作为发光层，进一步制备出基于无铅铜基卤化物薄膜的电致发光 LED 。然后利用HORIBA 荧光仪器对LED器件进行发光效率检测

CsPbBr3纳米片是以碳酸铯和溴化铅表面包裹上长碳链的有机配体形成前驱体，在溶剂热条件下(60−180 °C)合成的。另外，通过简单地改变铯油酸或溴化铅前驱体的加入量和加入顺序，可以实现CsPbBr3纳米片和Cs4PbBr6纳米晶之间的可逆化转变。研究中的发光测试，使用的是Fluorolog-3型荧光光谱仪。这套仪器的灵敏度、稳定性和分辨率都很高，为表征不同尺寸钙钛矿纳米片发光峰位红移提供了技术保障。

This particular investigation of phosphor emission characteristics was made possible by the excellent stray-light reflection, sensitivity, and wide lifetime range of the FLUOROLOG-τ® producing rapid and accurate characterization of the phosphor.