2020/09/21 11:03
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In this work we leveraged the analytical performance of the timsTOF Pro to identify expres-
sion of low-level enzymes for the first time in primary insulin-secreting beta cells (β-cells). We found that these proteins are critical, functional components of key metabolic pathways which
can support β-cell survival under inflammatory stress, an emerging disease etiology of diabetes. Confirmation that these factors are present and functional specifically in pancreatic β-cells points to new therapeutic avenues for human diabetes.
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4D-脂质组学高通量工作流程
Lipid profiling from complex lipid extracts can be a challenging and time consuming task. The high complexity of samples and co-elution of isobaric or isomeric compounds complicate the confident annotation of lipids. The presented 4D-Lipidomics workflow simplifies and streamlines the annotation and validation process using mobility-enhanced MS data.
医疗/卫生
2021/03/05
PASEF应用于COVID-19的高通量定量大规模血浆蛋白质组学研究
However, the dynamic range of protein concentration in these samples poses a challenge for in-depth proteome quantification. PASEF® is a powerful technology for rapid in-depth and sensitive proteome quantification that is also applicable to biofluids like plasma and serum. We combined PASEF with high-throughput gradient settings for the analysis of hundreds of plasma and serum samples and quantified several hundred protein groups routinely in 21- and 47-minute runtimes. The timsTOF Pro coupled with the Evosep One nanoflow UPLC delivers quantitation with median CV less than 20% and quantified more than 70 known biomarkers in a study investigating over 700 COVID-19 serum samples
医疗/卫生
2021/03/05
空间定位组学对肿瘤亚细胞群的深度蛋白组分析
MALDI Guided SpatialOMx,即基于质谱成像定位的空间多组学分析,完美的将MALDI成像技术和传统LC-MS/MS组学流程结合在了一起,它的特点是利用了MALDI成像技术能在分子原位对生物分子进行定位的特点,在生物组织的内部锁定目标微区(ROI,Region of Interests),在对该目标微区实施激光微切割(LCM,laser capture microdissection)后,进行LC-MS/MS组学分析,从而实现了深度的蛋白组信息挖掘。
医疗/卫生
2020/09/18
MALDI-2与timsTOF fleX的结合提升药物组织成像灵敏度
最新一代布鲁克timsTOF fleX使用创新的激光后电离MALDI-2技术获得了较传统MALDI 高1-3个数量级的灵敏度。MALDI-2提高了离子产率并降低了离子抑制效应。同时,MALDI-2还可对传统MALDI不能电离的药物和代谢物分子检测和成像,如甾醇、他汀类、激素、维生素、聚糖等生物分子。这种实质性的改进对于DMPK研究尤其重要。
医疗/卫生
2020/09/18