Special Petakas called “Easy Transfer” (Petaka ET) are manufactured with a set of paramagnetic micro-tools inside. These micro-tools (“surfers”), powered by the magnetic field created by a specific device (the Flipper), glide over the cells without scraping or shearing, lifting them intact using strictly “gentle physical forces” created by media vortex. Using Easy Transfer Petakas, ensures a clean cell release and suspension, avoiding cell damage from enzymes and surface molecules losses.

It is an environmental cell adaptation characterized by… the cell cycle slowing down, with cells in G1 & Mitosis passing through slowly to be arrested and gathered in the G2 phase………

传统的培养方式往往使得组织/细胞处于高氧环境，易产生自由式的ROS，从而导致DNA突变或者断裂、转录错误、蛋白合成受到影响等等，使得研究人员的实验数据产生偏差，如：研究细胞凋亡等

Disadvantages of the scrapers Upon observing the scraping edge under a microscope, it also reveals additional disadvantages... The scraper edges are large and rounded relative to the cell size and therefore, does not allows a real wiping action such as a snow plough, but acts more like a road roller crushing under the scraper blade the first line of cells .

Problems that come from culturing cells (mainly stem cells) in physiologically irrelevant O2 levels There are genes which are normally expressed in physiologic conditions in tissue cells, that in cell culture (21% O2) become suppressed and disappear or are significantly reduced

A frequent problem that arises when analyzing western blotting results is the presence of high background signal in the developed film or image. One potential source of such background signal is the buffers used for the dilution of the antibodies and blocking/washing of the membranes. While a variety of buffers are used in the process of antibody dilution and blocking/washing, different applications sometimes require a specific blocking and washing reagent and the amount of background levels can vary with them.

Many cell-based research studies require the counting of cells prior to beginning an experiment. This allows standardization of cell concentration between samples, minimizing error and variation in downstream results. The invention of the microscope and hemocytometer made cell counting possible, and is the most commonly used method to date. Fortunately, advancements in imaging technology have enabled the automation of cell counting, providing the best promise for improving experimental accuracy, reliability, and time-to-results, with much less time and effort. This technical note compares the Countess™ Automated Cell Counter to the hemocytometer method of cell counting, examining methodology, accuracy, precision, and dynamic range. The Countess™ method provides comparable accuracy and precision to the most accurate hemocytometer method, while offering the following additional benefits: •• Simple, automated procedure •• Faster time-to-results •• Broader concentration range measured

Many cell-based research studies require the counting of cells prior to beginning an experiment. This allows standardization of cell concentration between samples, minimizing error and variation in downstream results. There are several different counting methods and instruments available to accomplish this task, including glass and disposable hemocytometers, flow cytometers, and benchtop counters. This technical note compares the Countess™ Automated Cell Counter to the Beckman Coulter Z1™ Series Coulter Counter® Cell and Particle Counter, examining methodology and performance. The Countess™ method provides comparable performance to the Coulter counter method, while offering the following additional benefits: ••Easier methodology, setup, and maintenance ••Provides cell viability and size data in addition to cell counts ••Actual results are visualized, increasing confidence ••More affordable

Many cell-based research studies require the counting of cells prior to beginning an experiment. This allows standardization of cell concentration between samples to minimize error and variation in downstream results. Several different cell counting instruments are available: •• Glass and disposable hemocytometers •• Flow cytometers •• Benchtop counters

为了让客户更充分了解自动细胞计数仪的功能和目前的使用情况，特罗列如下几篇文献仅供参考： •Comparison Vi-CELL® XR Cell Viability Analyzer •Counting Blood Cells

为了满足客户在使用自动细胞计数器时遇到的各种问题，特总结了各种常见问题！ Q How long does it take to count? A 30 seconds per sample

等电聚焦工作模式:最高可达0.01PH,用于分离蛋白质和多肽； 区带电泳工作模式：用于分离细胞器、生物粒子和细胞；

2D胶作为目前蛋白质组研究必不可少的工具之一，虽然应用很普遍，但是存在着固有的缺陷： 1） 重复性低，同样的样品，同一个操作者，重复性只有70％； 2） 做质谱需要进行样品回收，回收率大约只有30％； 3） 所需时间长，需要过夜。PRIEF3000具有高重复性、高回收率，操作时间短等优点，这是传统的2D胶所无法比拟的。对于需要进行蛋白鉴定的客户，或许他们会选择购买PRIEF3000。