To demonstrate the rapid quantitation of a low-level human IgG in animal
plasma by LC/MS/MS using the Thermo Scientific™ SMART Digest™
Immunoaffinity (IA) kits (including magnetic and non-magnetic versions of the
Streptavidin kit, Protein A kit, and Protein G kit), which combine the IA capture
and digestion process into a single well. The assay is required to be both
selective and accurate.
? The rapid processing of antibodies using the SMART
Digest IA kits allows development of faster methods
compared to conventional protein enrichment and
digestion by simplifying sample preparation and
enabling a reproducible, sensitive, and fully integrated
? This application shows a rapid, simple, and sensitive
method for the quantification of human IgG in mouse
plasma using the SMART Digest IA kits with a total time
of 3–4 hours that compares to a process that can take
up to 24 hours.
? The method described provides a significant
simplification for the affinity proteomics process within
a single well providing increased sensitivity and
reduced sample preparation time.
? This Application Note provides a general approach for
quantifying low abundance mAbs with reproducible
results across a broad dynamic range for all three types
of SMART Digest (IA) kits (SMART digest IA
Streptavidin, Protein A, and Protein G).