Ogataea polymorpha

平台编号：Bio-135130

Ogataea polymorpha (Morais et Maia) Yamada, Maeda et Mikata

34438™

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An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.

Product category

Fungi

Product type

Yeast

Strain designation

NRRL Y-5445 [CBS 4732, CCRC 20467, DSM 70277, IFO 1476, JCM 3621, MUCL 27761, NCYC 1457]

Type strain

Yes

Genome sequenced strain

Yes

Isolation source

Soil

Geographical isolation

Brazil

Applications

Bioremediation

Biofuel production

Product format

Frozen

Storage conditions

-80°C or colder

General

Specific applications

degrades xylose D-xylose

produces acetone reductase

produces alcohol dehydrogenase

produces alcohol oxidase

produces glycerol 2-dehydrogenase (NADP) dihydroxyacetone reductase

produces single-cell protein SCP

procudes copper-containing amine oxidase

produces SCP from xylose

Potential bioremediation agent: lignin

Preceptrol

No

Characteristics

Comments

catalase activity following growth on methanol

Genome sequencing strain (Genolevures Consortium, France).

Handling information

Medium

ATCC Medium 200: YM agar or YM broth

ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth

ATCC Medium 1245: YEPD

Temperature

24-26°C

Atmosphere

Aerobic

Handling procedure

Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen.  If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week.  Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C).  Storage of frozen material at this temperature will result in the death of the culture.

To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes).  Immerse the ampoule just sufficient to cover the frozen material.  Do not agitate the ampoule.

Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer 10 µL (or any amount desired up to all) of the content onto a plate or broth with medium recommended.

Incubate the inoculum/strain at the temperature and conditions recommended.

Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain. 

Handling notes

Type strain; degrades xylose D-xylose; produces acetone reductase; produces alcohol; dehydrogenase; produces alcohol oxidase; produces glycerol 2-dehydrogenase (NADP) dihydroxyacetone reductase; produces single-cell protein SCP; procudes copper-containing amine oxidase; produces SCP from xylose; related products - genomic DNA (ATCC 34438D-5)

Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications