|
Feature |
Benefit |
|
Sensitive to endogenous receptors |
Enables reliable and robust measurements of endogenous cellular receptor activity, even low density receptors which may be undetectable with other functional assays. |
|
Label free detection |
Expensive tags, dyes, or specialized reagents are not required. This reduces the resources required for assay development and eliminates the incidence of undesirable interactions with labels. |
|
Universal platform for functional cell surface target assays |
Single platform and assay methodology measures GPCRs, TKRs, adhesion molecules, and indirectly measures ligand-gated ion channels, among other targets. |
|
Measures all cell types |
System measures disease and biologically relevant cell lines and primary cells, including adherent and non-adherent cell types. |
|
Measures all classes of compound efficacy |
Comprehensive detection of agonists, antagonists, partial and inverse agonists, and allosteric modulators in a native setting in a single assay. |
|
Information-rich kinetic response data |
Reproducible and robust qualitative pathway-specific cell response profiles. Probe complex receptor signaling, pathway diversity, and identify the underlying mechanism of action (MOA) of lead compounds. |
|
Streamlined assay development |
Single-assay format for multiple receptor types regardless of signal coupling mechanism. Reduces the resources spent on assay development, such as time consuming and costly biological manipulation of cell lines and the optimization of labeling reagents. |
|
Integrated, internal pipettor head |
Simultaneous addition of ligand and/or compound while measuring the fast kinetic cellular responses, delivers reproducible and robust response profiles, with no loss of information. |
|
Automation-friendly |
Designed for easy robotic integration with small and large scale automation platforms. |
|
Ease of use |
The CellKey System is a fully integrated, flexible, and intuitive platform. |
|
Measurements |
|
|
Assay type: |
Homogeneous, label-free, cell-based |
|
Principles: |
Impedance |
|
Frequency range: |
1 kHz-10 MHz |
|
Mode: |
Kinetic measurement: simultaneous compound addition and measurement across all wells |
|
Data acquisition rate: |
2 or 10 second update rate |
|
Microplate Formats |
|
|
Cell plate format: |
96-well proprietary cell plate |
|
Compound plate format: |
96-well and 384-well compound plates and reservoirs |
|
Deck positions: |
One read (cell plate) position Two compound plate positions One tip loading position |
|
Fluidics |
|
|
Pipettor format: |
One 96 well pipettor head, simultaneous transfer |
|
Pipettor range: |
0.5-200 μL |
|
Pipetting increments: |
0.1 μL |
|
Dispense speed: |
Adjustable |
|
Pipettor height: |
Adjustable |
|
Additional standard features: |
Compound mix capability |
|
Throughput |
|
|
Assay development: |
4 plates/hour (15-minute read time) |
|
High-throughput: |
8 plates/hour or better (5-minute read time, plus automation) |
|
Temperature Range |
|
|
Analysis range: |
Ambient +5° to 37°C (below 20°C, ambient +6°) |
|
Ambient range: |
15-30oC |
|
Physical Characteristics |
|
|
Benchtop |
Yes |
|
Instrument weight: |
128 kg/282 lbs |
|
Instrument dimensions (in): |
28 (H) x 33 (W) x 23 (D) |
|
Instrument dimensions (cm): |
70 (H) x 85 (W) x 58 (D) |
|
Power consumption: |
630 W |
|
Power source: |
100-120/200-240 V (self-adjusting) 50/60 Hz |
|
Computer |
|
|
Processor: |
Minimum: Intel® Pentium® 4 3.6GHz processor, 1GB RAM |
|
LCD monitor: |
20-inch |
|
Operating system: |
Windows® XP |
|
Regulatory |
|
|
Regulatory: |
For Research Use Only. Not for Use in diagnostic procedures |
