自动细胞计数器文献

Special Petakas called “Easy Transfer” (Petaka ET) are manufactured with a set of paramagnetic micro-tools inside. These micro-tools (“surfers”), powered by the magnetic field created by a specific device (the Flipper), glide over the cells without scraping or shearing, lifting them intact using strictly “gentle physical forces” created by media vortex. Using Easy Transfer Petakas, ensures a clean cell release and suspension, avoiding cell damage from enzymes and surface molecules losses.

It is an environmental cell adaptation characterized by… the cell cycle slowing down, with cells in G1 & Mitosis passing through slowly to be arrested and gathered in the G2 phase………

传统的培养方式往往使得组织/细胞处于高氧环境，易产生自由式的ROS，从而导致DNA突变或者断裂、转录错误、蛋白合成受到影响等等，使得研究人员的实验数据产生偏差，如：研究细胞凋亡等

Disadvantages of the scrapers Upon observing the scraping edge under a microscope, it also reveals additional disadvantages... The scraper edges are large and rounded relative to the cell size and therefore, does not allows a real wiping action such as a snow plough, but acts more like a road roller crushing under the scraper blade the first line of cells .