细胞培养-细胞收获

Special Petakas called “Easy Transfer” (Petaka ET) are manufactured with a set of paramagnetic micro-tools inside. These micro-tools (“surfers”), powered by the magnetic field created by a specific device (the Flipper), glide over the cells without scraping or shearing, lifting them intact using strictly “gentle physical forces” created by media vortex. Using Easy Transfer Petakas, ensures a clean cell release and suspension, avoiding cell damage from enzymes and surface molecules losses.

It is an environmental cell adaptation characterized by… the cell cycle slowing down, with cells in G1 & Mitosis passing through slowly to be arrested and gathered in the G2 phase………

传统的培养方式往往使得组织/细胞处于高氧环境，易产生自由式的ROS，从而导致DNA突变或者断裂、转录错误、蛋白合成受到影响等等，使得研究人员的实验数据产生偏差，如：研究细胞凋亡等

Problems that come from culturing cells (mainly stem cells) in physiologically irrelevant O2 levels There are genes which are normally expressed in physiologic conditions in tissue cells, that in cell culture (21% O2) become suppressed and disappear or are significantly reduced