肝素酶(HPA)多克隆抗体Mouse

肝素酶(HPA)多克隆抗体Polyclonal Antibody to Heparanase (HPA)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

肝素酶(HPA)多克隆抗体ANTIBODY SPECIFITY

The antibody is a rabbit polyclonal antibody raised against HPA. It has been selected for its ability to recognize HPA in immunohistochemical staining and western blotting.

APPLICATIONS

Western blotting: 1:50-400 
Immunocytochemistry in formalin fixed cells: 1:50-500 
Immunohistochemistry in formalin fixed frozen section: 1:50-500 
Immunohistochemistry in paraffin section: 1:10-100 
Enzyme-linked Immunosorbent Assay: 1:100-200 
Optimal working dilutions must be determined by end user. 

CONTENTS

Form & Buffer: Supplied as solution form in PBS, pH7.4, 
containing 0.02% NaN3, 50% glycerol.

IHC Images

肝素酶(HPA)多克隆抗体Used in DAB staining on fromalin fixed paraffin- embedded oviduct tissue

QUALITY CONTROL

Content: The quality control contains recombinant HPA (Glu280~Leu412) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

STORAGE

Store at 4^oC for frequent use. Stored at -20^oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

肝素酶(HPA)多克隆抗体

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