获取电话Anti-CD23 antibody
种属反应性Human,Mouse,Rat,Horse,Rabbit
验证应用WB,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human CD23 221-321/321
偶联Non-conjugated
Anti-CD23 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Cell membrane; Single-pass type II membrane protein. Secreted. Note=Also exists as a soluble excreted form.
其它名称
moreAnti-CD23 antibody应用
WB:1:500-2000
FC:1μg/Test
Fig1: Blank control(blue):Raji cells (fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti- CD23 antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Fig2: Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti- CD23 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 50/40 kD
Fig3: Sample:
293T Cell (Human) Lysate at 30 ug
Primary: Anti- CD23at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 50/40 kD
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