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一键询价Anti-CD108 antibody
种属反应性Human,Mouse,Rat,Dog,Cow
验证应用WB,IHC-P
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human Sema7A 351-450/666
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side. Note=Detected in a punctate pattern on the cell membrane of basal and supra-basal skin keratinocytes.
其它名称
WB:1:500-2000
IHC-P:1:400-800
Fig1: Sample:
Lane 1: Brain (Mouse) Lysate at 30 ug
Lane 2: Lung (Mouse) Lysate at 30 ug
Primary: Anti-Sema7A/CD108at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG at 1: 3000 dilution;
Predicted band size : 66kD
Observed band size : 66kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CD108) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
Fig3: Sample:
Cerebrum (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Primary: Anti-CD108 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kD
Observed band size: 63 kD
Fig4: Sample:
Skin (Mouse) Lysate at 40 ug
Skin(Rat) Lysate at 40 ug
Primary: Anti-CD108 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 66 kD
Observed band size: 66 kD
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!