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Anti-Rad21 antibody

Anti-Rad21 antibody

价格: 1380

品牌:泽叶生物

供货周期: 现货

货号:ZY-6803-73R

规格:兔多抗

Anti-Rad21 antibody

  • 种属反应性Human,Mouse,Rat

  • 验证应用WB,ICC,IHC-P

  • 抗体类型兔多抗

  • 免疫原Recombinant protein within human Rad21 aa 250-440.

  • 偶联Non-conjugated

  • Anti-Rad21 antibody性能

  • 形态Liquid

  • 浓度1 mg/ml.

  • 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

  • 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • 亚型IgG

  • 纯化方式Protein affinity purified.

  • 亚细胞定位Centromere, Chromosome, Nucleus.

  • 其它名称CDLS4 antibody
    Double-strand-break repair protein rad21 homolog antibody
    hHR21 antibody
    HR21 antibody
    HRAD21 antibody
    KIAA0078 antibody
    MCD1 antibody
    Nuclear matrix protein 1 antibody
    NXP-1 antibody
    NXP1 antibody
    Protein involved in DNA double-strand break repair antibody
    RAD21 antibody
    RAD21 homolog (S. pombe) antibody
    RAD21 homolog antibody
    RAD21_HUMAN antibody
    Scc1 antibody
    SCC1 homolog antibody

    more
  • Anti-Rad21 antibody应用

    WB:1:500-1:2,000
    ICC:1:50-1:200
    IHC-P:1:100-1:400

  • Fig1: Western blot analysis of Rad21 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Mouse ovary tissue lysate
    Lane 2: Daudi cell lysate

    Fig2: ICC staining Rad21 in MG-63 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Rad21 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

    Fig3: ICC staining Rad21 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Rad21 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

    Fig4: ICC staining Rad21 in SK-Br-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Rad21 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

    Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Rad21 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-73) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

    Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer tissue using anti-Rad21 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-73) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

    Fig7: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Rad21 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA

    Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Rad21 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA

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生化试剂 Anti-Rad21 antibody由上海泽叶生物科技有限公司为您提供,如想了解更多关于抗体/抗原 Anti-Rad21 antibody的报价、规格、厂家等信息,欢迎来电或留言咨询。除供应Anti-Rad21 antibody外,上海泽叶生物科技有限公司还可为您提供: 彩色预染蛋白Marker(10-180 kDa,三色)、 Super GelBlueTM 核酸染料, 10,000× in water 、 彩色预染蛋白Marker(10-180 kDa,三色)
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