获取电话Anti-delta 1 Catenin/CAS antibody
种属反应性Human,Rat
验证应用WB,ICC
抗体类型兔多抗
免疫原Recombinant protein within human delta 1 Catenin/CAS aa 130-320.
偶联Non-conjugated
Anti-delta 1 Catenin/CAS antibody性能
形态Liquid
浓度1 mg/ml.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Protein A purified.
亚细胞定位Cell membrane, Cytoplasm, Membrane, Nucleus. Interaction with GLIS2 promotes nuclear translocation (By similarity). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm.
数据链接SwissProt: O60716 Human
其它名称
moreAnti-delta 1 Catenin/CAS antibody应用
WB:1:500
ICC:1:50-1:200Fig1: Western blot analysis of delta 1 Catenin/CAS on A431 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: ICC staining delta 1 Catenin/CAS in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with delta 1 Catenin/CAS polyclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Fig3: ICC staining delta 1 Catenin/CAS in PMVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with delta 1 Catenin/CAS polyclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Fig4: ICC staining delta 1 Catenin/CAS in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with delta 1 Catenin/CAS polyclonal antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
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