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Anti-EGFR antibody

Anti-EGFR antibody

价格: 1080

品牌:泽叶生物

供货周期: 现货

货号:ZY-0407R

规格:50μl

Anti-EGFR antibody

  • 种属反应性Human,Mouse,Rat

  • 验证应用WB,ICC,IHC-P,FC

  • 抗体类型兔多抗

  • 免疫原This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of N-terminal residues of mouse EGFR.

  • 偶联Non-conjugated

  • 性能

  • 形态Liquid

  • 浓度1 mg/mL.

  • 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

  • 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • 亚型IgG

  • 纯化方式Peptide affinity purified.

  • 亚细胞定位Plasma membrane, Nucleus, Endosome, Endoplasmic reticulum.

  • 数据链接SwissProt: P00533 Human
    SwissProt: Q01279 Mouse
    Entrez Gene: 24329 Rat

  • 其它名称Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
    Cell growth inhibiting protein 40 antibody
    Cell proliferation inducing protein 61 antibody
    EGF R antibody
    EGFR antibody
    EGFR_HUMAN antibody
    Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
    Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
    Epidermal growth factor receptor antibody
    erb-b2 receptor tyrosine kinase 1 antibody
    ERBB antibody
    ERBB1 antibody
    Errp antibody
    HER1 antibody
    mENA antibody
    NISBD2 antibody
    Oncogen ERBB antibody
    PIG61 antibody
    Proto-oncogene c-ErbB-1 antibody
    Receptor tyrosine protein kinase ErbB 1 antibody
    Receptor tyrosine-protein kinase ErbB-1 antibody
    SA7 antibody
    Species antigen 7 antibody
    Urogastrone antibody
    v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
    wa2 antibody
    Wa5 antibody

    more
  • 应用

    WB:1:500-1:1,000
    ICC:1:50-1:200
    IHC-P:1:50-1:200
    FC:1:50-1:100

  • Anti-EGFR antibody

  • Fig1: Western blot analysis of EGFR on A431 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig2: ICC staining EGFR in A172 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-21) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

    Fig3: Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-EGFR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (0407-21) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

    Fig4: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-EGFR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (0407-21) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

    Fig5: Flow cytometric analysis of EGFR was done on A431 cells. The cells were fixed, permeabilized and stained with EGFR antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for at least 30 minutes.


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生化试剂 Anti-EGFR antibody由上海泽叶生物科技有限公司为您提供,如想了解更多关于抗体/抗原 Anti-EGFR antibody的报价、规格、厂家等信息,欢迎来电或留言咨询。除供应Anti-EGFR antibody外,上海泽叶生物科技有限公司还可为您提供: 彩色预染蛋白Marker(10-180 kDa,三色)、 Super GelBlueTM 核酸染料, 10,000× in water 、 彩色预染蛋白Marker(10-180 kDa,三色)
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