获取电话Anti-CD4 antibody
种属反应性Human,Mouse,Rat
验证应用WB,ICC,FC
抗体类型兔多抗
免疫原This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to mouse CD4 aa 200-250.
偶联Non-conjugated
Anti-CD4 antibody性能
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Cell Membrane
数据链接P01730 Human
P06332 Mouse
P05540 Rat其它名称
moreAnti-CD4 antibody应用
WB: 1:500
ICC: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of CD4 on mouse brain lysate using anti-CD4 antibody at 1/500 dilution.
Fig2: ICC staining CD4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Flow cytometric analysis of Raji cells with CD4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
