细胞裂解直接到RT-qPCR试剂盒，探针法

这个是探针法的两步法。SingleShot 家族产品是将细胞裂解后，不经过RNA/DNA纯化，即可上样进行RT或qPCR反应，直接得到结果。涵盖细胞裂解液， RT试剂，qPCR试剂。 包含一步法RT-qPCR试剂盒或两步法试剂盒即RT先做然后再做qPCR。裂解也可单独销售。包含探针法和染料法。 非常适合高通量细胞培养时进行基因表达检测，利于药筛实验。也适合细胞数量非常少的样品，不方便进行核酸抽提，可使用此试剂盒直接进行细胞裂解到定量扩增，利于低至单细胞的样品。 SingleShot? Cell Lysis RT-qPCR Kits provide a complete and fast solution for the generation of lysates from cell cultures, optimized for downstream one step or two step quantitative PCR (qPCR) reactions, without an RNA purification step.Single Shot Workflow Kits are available in SYBR? Green or probe chemistries. A cell lysis kit is also offered as a stand-alone option. All kits offer the following key benefits: Cell lysate from 10 to 100,000 cells ready in 20 minutes Complete removal of genomic DNA while preserving RNA integrity Optimal accuracy and linear dynamic range of qPCR data across genes Results comparable to using purified RNA, including dynamic range