搜全网
搜本展位

布鲁克 timsTOF™ Pro 捕集离子淌度质谱

产品详情

布鲁克 timsTOF™ Pro 捕集离子淌度质谱

布鲁克 timsTOF™ Pro 捕集离子淌度质谱
参考报价:
 面议
型号:
 timsTOF Pro
品牌:
 布鲁克
产地:
 德国
样本:  【下载】 信息完整度:  
典型用户:  0
产地类别:
 进口仪器
质量范围:
 
灵敏度:
 
分辨率:
 
精确质量数:
 
价格区间:
 500万-750万
仪器种类:
 四极杆-飞行时间
分享:
400-860-5168转0230
仪器信息网认证,请放心拨打
仪信通金牌会员 第 18 年
仪信通诚信认证
仪器信息网认证,请放心拨打
400-860-5168转0230
名 称: 布鲁克·道尔顿(Bruker Daltonics)
认 证: 工商信息已核实
信用积分:4112
关注我们
关注我们
布鲁克·道尔顿手机版
产品简介

the timsTOF™ Pro Mass Spectrometer Enables the Revolutionary PASEF Method for Next-Generation Proteomics

Unique Trapped Ion Mobility Spectrometry (TIMS) Adds New Dimension for Robust, Faster Proteome Analysis with Higher Sensitivity

Overview

  • Powered by PASEF

Mass spectrometry (MS)-based proteomics has become a powerful technology for the identification and quantification of thousands of proteins. However, the coverage of complete proteomes is still very challenging due to the limited speed, sensitivity and resolution of current mass spectrometers.
The timsTOF Pro uses the Parallel Accumulation Serial Fragmentation (PASEF) acquisition method to provide extremely high speed and sensitivity to reach new depths in shotgun proteomics and phosphoproteomics using low sample amounts.

  • Achieve near 100% duty cycle for shotgun proteomics

A near 100% duty cycle for high sensitivity, high speed shotgun proteomics can now be achieved with the dual TIMS technology. The novel design allows for ions to be accumulated in the front section, while ions in the rear section are simultaneously released depending on their ion mobility. This process is called Parallel Accumulation Serial Fragmentation or PASEF.

  • PASEF digs deeper into the proteome

The timsTOF Pro powered by PASEF offers sequencing speed > 100 Hz without losing sensitivity by synchronizing the quadrupole isolation mass window with the elution time of the specific peptide packages from the TIMS funnel.

  • MaxQuant/Perseus and PEAKS Studio enabled data processing

An open-file data format allows researchers to directly work with the raw data and use industry leading software. MaxQuant has been adapted to manage 4D features in the space spanned by retention time, ion mobility, mass, and signal intensity which benefit the identification and quantification of peptides, proteins, and posttranslational modifications. PEAKS Studio combines de novo sequencing with tradtional databbase searches and is optimized for processing timsTOF raw data.


Technical Details

  • Outstanding hardware performance

The second generation dual TIMS analyzer is optimized for shotgun proteomics needs. Due to its unique geometry ions are released dependent on their mobility from the second section of the TIMS analyzer while the further incoming ions can be accumulated in parallel in the first part of the TIMS analyzer. With the parallel accumulation technology a duty cycle up to 100% can be achieved resulting in no ion loss.

In the timsTOF Pro an advanced segmented quadrupole mass filter is used for high ion transmission and isolation efficiency. The quadrupole mass position is synchronized with the elution times of the specific ions from the TIMS analyzer. Due to its ultra-high mass position switching time (< 1 ms) it enables the best performance for the PASEF method.

  • TIMS

Trapped Ion Mobility Spectrometry (TIMS) is first and foremost a separation technique in gas phase, which resolves sample complexity with an added dimension of separation in addition to HPLC and mass spectrometry, increasing peak capacity and confidence in compound characterization.

  • PASEF

With speed in mind, Bruker experts redesigned MS/MS technology to meet the requirements of shotgun proteomics. Peptide ions are separated using trapped ion mobility spectrometry, eluted (~ 100 ms) and detected in the QTOF, generating the TIMS MS heat map (A). In the PASEF method (B) the same TIMS separation is used with the quadrupole isolating a certain ion species during its elution and immediately shifting to the next precursor. Parent and fragment spectra are aligned by mobility values. With the Parallel Accumulation Serial Fragmentation (PASEF) Technology >100 Hz sequencing speed can be achieved. With the PASEF method the MS/MS spectra quality of the low abundant peptides can be increased by selecting them several times.


相关产品
布鲁克scimaX™
型号:scimaX™
布鲁克rapifleX MALDI
型号:rapifleX MALDI Tissu
solariX XR傅里叶变换
型号:solariX XR
autoflex maX MALDI-T
型号:autoflex maX
布鲁克impact II四极
型号:impact II
布鲁克timsTOF捕集离
型号:timsTOF
  • 推荐产品
  • 供应产品
  • 产品分类
400-860-5168
分机号:0230
仪器信息网认证,
请放心拨打
访问手机展位
布鲁克·道尔顿官方展位由仪器信息网设计制作,工商信息已核实。
展位地址:https://www.instrument.com.cn/netshow/SH100230/