The novel column chemistry of the Acclaim® Mixed-Mode WAX-1 results in a multimode separation mechanism including hydrophobic, anion-exchange, and cation-exclusion interactions. Consequently, retention of basic, neutral, and acidic molecules can be either independently or concurrently adjusted by changing ionic strength, pH, and organic solvent content in the mobile phase.
Adjustable selectivity
Selectivity orthogonal to reversed-phase (RP) columns
Ideal selectivity for anionic molecules
Simultaneous separation of acidic, basic, and neutral molecules
Multimode retention mechanisms: reversed- and normal phase, anion-exchange, cation-exclusion, and HILIC modes
The Acclaim Mixed-Mode WAX-1 column combines hydrophobic and anion-exchange characteristics, on a 5 μm high-purity, porous silica with 120-? diameter pores. Because of this unique chemistry, the Acclaim Mixed-Mode WAX-1 column also provides complimentary selectivity to C18 reversed-phase columns.
Hydrophilic organic acids are often difficult to separate with common RP columns, since hydrophobic retention alone is inadequate to differentiate molecules with similar hydrophobicities. This can be overcome using the Acclaim Mixed-Mode WAX-1 column; it provides sufficient retention, and ideal selectivity for a variety of anionic molecules, even for weakly charged molecules.
Retention of basic, neutral and acidic molecules can be either independently or concurrently adjusted by changing ionic strength, pH, and organic solvents content in the mobile phase. The presence of anion-exchange functionality control through electrostatic attraction (anions), repulsion (cations). The alkyl chain controls separation by hydrophobic interaction.
A broad range of samples in the pharmaceutical, food and beverage and chemical industries can be separated. Separation of basic active ingredients and its counter-ion can be simultaneously determined, as well as separation of ingredients containing basic, neutral, and acidic ingredients.
| Acclaim Mixed-Mode WAX-1 Columns: A simple solution to difficult separations Specifications | |
|---|---|
| Column Chemistry | Alkyl amine |
| Base silica | Spherical, high-purity |
| Particle size | 5 μm |
| Pore size | 120 ? |
| Surface area | 300 m2/g |
| Analytical Columns | |
|---|---|
| Acclaim Mixed-Mode WAX-1, 3μm Analytical, (3.0 x 150mm) | 070088 |
| Acclaim Mixed-Mode WAX-1, 3μm Analytical, (3.0 x 50mm) | 071908 |
| Acclaim Mixed-Mode WAX-1, 3μm Analytical, (2.1 x 150mm) | 070089 |
| Acclaim Mixed-Mode WAX-1, 5 μm (4.6 x 150 mm) | 064984 |
| Acclaim Mixed-Mode WAX-1, 5 μm (4.6 x 250 mm) | 064985 |
| Acclaim Mixed-Mode WAX-1, 5 μm, 2.1 x 150 mm | 067084 |
| Guard Columns | |
|---|---|
| Acclaim Mixed-Mode WAX-1, 5 μm Guard Cartridges (4.6 x 10 mm), 2 ea (use V-2 Holder) | 069704 |
| Acclaim Mixed-Mode WAX-1, 5μm Guards (3 x 10mm), 2ea, requires holder 069580 | 071909 |
| Acclaim Mixed-Mode WAX-1, 5μm, Guards (2.1 x 10mm), 2 ea; requires holder 069580 | 069686 |
| Acclaim SST Guard Cartridge Holder V-2 | 069580 |
| Acclaim Guard Kit (Holder and coupler) V-2 | 069707 |
| Guard to Analytical Column Coupler V-2 | |
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