Efficiently resolve oligosaccharides released from therapeutic glycoproteins differing in size, charge, branching, and linkage with the Thermo Scientific™ Dionex™ CarboPac™ PA100 Analytical & Guard Columns. It provides the flexibility to separate oligosaccharides into broad classes depending upon the degree of sialylation, while maintaining the resolution needed for routine analysis based on linkage isomerism. Coupled with pulsed amperometric detection (PAD), this high-resolution, strong anion-exchange column supports detection at low-picomole levels for enhanced chromatography of oligosaccharides.
The resin of Dionex CarboPac PA100 Columns consists of 5.5μm diameter nonporous beads covered with a fine layer of functionalized Thermo Scientific™ Dionex™ MicroBead™ latex. This pellicular resin structure permits excellent mass transfer, resulting in high-resolution chromatography and rapid re-equilibration. The 3 x 250mm format provides fast separations, and the 0.5mL/minute recommended flow rate results in significant savings in eluent consumption.
Predictable, High-Resolution Separations
Separates closely related oligosaccharides, even isomers, at picomole levels
Separates neutral and charged oligosaccharides in the same run
Requires no sample derivatization for detection
Use the Thermo Scientific™ Dionex™ CMD™ Carbohydrate Membrane Desalter on line prior to fraction collection to provide easy, automated removal of eluent salts.
About the Dionex CarboPac Family of Columns
The Dionex CarboPac family offers a selection of high-performance liquid chromatography (HPLC) columns, each optimized for a different class of compounds. Combined with PAD, which provides a means to detect carbohydrates without derivatization, these columns support simple, reliable techniques to separate sugar alcohols, mono- and disaccharides, and poly- and oligosaccharides.
Dionex CarboPac columns provide high-resolution separations of:
Glycoprotein oligosaccharides and complex carbohydrates from dietary fiber, including fructans, maltodextrins, and amylopectins
Monosaccharides, such as those obtained from glycoprotein hydrolysates or plant hydrolysates
Mono- and disaccharides
| 货号 | 产品规格 | 类型 | 直径（公制） | 长度（公制） | 单价 (CNY) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 043055 | - | Standard Bore Separator | 4mm | 250mm | 申请报价 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 产品规格 | - |
|---|---|
| 适用于 | HPAE-PAD |
| 直径（公制） | 4mm |
| 流速 | 1.0mL/min |
| 最大压力 | 4000psi |
| 流动相兼容性 | 100% compatible with common organic solvents; ionic form eluents: sodium acetate and sodium hydroxide only. |
| 粒径 | 8.5μm |
| pH | 0-14 |
| 色谱柱容量 | 90μeq |
| 长度（公制） | 250mm |
| 技术. | Carbohydrate Analysis |
| 柱结构 | PEEK |
| 树脂组成 | 8.5μm diameter ethylvinylbenzene/divinylbenzene substrate (55% cross-linking) agglomerated with 275nm MicroBead quaternary amine functionalized latex (6% cross-linked). |
| 固定相 | CarboPac PA100 |
| 温度 | 4° to 60°C |
| 类型 | Standard Bore Separator |
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