使用带有微采样盘的OneDrop附件测量有机化合物和金属配合物的CD光谱

虽然CD的测量通常用于分析蛋白质的二级结构，但CD也可以用来确定有机化合物的构型和金属配合物与光学活性配体的配位。结论该应用说明表明，一滴微采样盘为体积有限样品的CD光谱测量提供了可靠的光谱。Application NoteCDSSpectroscopy One-Drop CD spectra measurements oforganic compounds and metal-complexes2/3Application Note One-Drop CD spectra measurements of organic compounds and metal-complexes using a microsampling disc Introduction Whi l e C D meas u reme n ts a r e t yp i cal l y ob t a i ned t o a n a l yse the seco n d a r y stru c tu re o f pr o tein s， CD ca n a l s o b e u se d t o d et e rmin e the c on fi g ura ti o n of o r g an i c co mp o u n ds an d coor d inat i on of me t a l comple x es w ith opti c ally active l i gands. Th is appl i catio n no t e wi ll com p are C D s p ectra obtained with the On e-Dr o p mi c ro sampli ng dis k a n d a co nve n ti o nal c e l l fo r o rg a n i c c o mp o u nd s w ith a n ab so r pti o n b a n d i n the u ltr av i ole t (UV) re g ion and metal complexes w ith an ab s o rp tio n b a n d in th e UV -V i si bl e re g i o n . JASCO J -1500 CD spectrometer View product i nformation at w ww .jas co i n c.c OM Keywords On e-Dr o p m eas u re m en t ， m i c r osamp l i ng disk， ci rc u l ar d i c h roism ， o r ga n ic compou n ds ， metal complexes ， J -1500 Experimental Measurement conditions for organic compounds (Figure 1) J-1500 CD spectrometer， MSD， conventional cell Spectral bandwidth 1nm Accumulation 4 times (MSD)， 1 time (conventional cell) Data acquisition interval 0.1nm Response time 2 seconds Scan speed 100 nm/min Sample volume 10 uL (MSD)， 400 uL (conventional cell) Path length 1mm Measurement conditions for metal complexes (Figure 2) J-1500 CD spectrometer， MSD， conventional cell Spectral bandwidth 1nm Accumulation 9 times (MSD)， 1 time (conventional cell) Data acquisition interval 0.1nm Response time 2 seconds Scan speed 200 nm/min Sample volume 10 pL (MSD)， 400 uL (conventional cell) Path length 1mm Results The CD s pec tra de pi cted in Fi g ures 1 a n d 2 c omp a re the result s o b t a i n e d us in g t he MSD a nd a con v en ti o n al ce l l fo r o r ga n ic c o m p o u nd s an d metal c o mplexes ， res p ecti v e ly. B oth the or gan i c co m p o u nd s an d m e t a l co m pl exe s' d at a sh ow good corre l at i on be t ween s p ectra measu r ed with th e 10 pL volu m e mic r osamp lin g disk (blu e ) a n d 400 p L vol u me con v e n tion a l cel l (g r ee n )， ea c h with a 1 mm p a th l e n g th . Figure 1. C D s p e ctr a of (A) (1S)-(+)-10-C a m p ho r su l fo ni d a c i d ， ammou n i u m salt an d (B ) D-Pa n t o l a c to ne us in g a m i c r osamplin g d isk (bl u e) and cylin dri ca l ce l l (g r ee n). 28600 Mary's C o u r t， Easto n， MD 21601 USA Figure 2. C D s p ectr a of (A) 2(+)D-[C oen 3]CI3NaCI -6H 2O an d (B) 0.25 M N iSO4+， 0.36 M Ro c h el l e sa l t u sin g a mi c r osampli n g d i s k (bl u e) an d c ylin dri c al c e l l (g re e n ).