动物血浆中治疗性抗体检测产品配置单(液相色谱仪)

  • 应用领域: 医疗/卫生
  • 检测样品: 动物血浆
  • 检测项目:治疗性抗体
  • 浏览次数:15 次
  • 下载次数:0 次
  • 发布时间:2018-03-13
  • 参考标准:SMART Digest IA USERS MANUAL: https://tools.thermofisher.com/content/sfs/manuals/Man-21549-SMART-Digest-IA-User-Man21549-EN.pdf
• The rapid processing of antibodies using the SMART Digest IA kits allows development of faster methods compared to conventional protein enrichment and digestion by simplifying sample preparation and enabling a reproducible, sensitive, and fully integrated LC/MS/MS workflow. • This application shows a rapid, simple, and sensitive method for the quantification of human IgG in mouse plasma using the SMART Digest IA kits with a total time of 3–4 hours that compares to a process that can take up to 24 hours. • The method described provides a significant simplification for the affinity proteomics process within a single well providing increased sensitivity and reduced sample preparation time. • This Application Note provides a general approach for quantifying low abundance mAbs with reproducible results across a broad dynamic range for all three types of SMART Digest (IA) kits (SMART digest IA Streptavidin, Protein A, and Protein G).
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  • 参考报价

To demonstrate the rapid quantitation of a low-level human IgG in animal
plasma by LC/MS/MS using the Thermo Scientific™ SMART Digest™
Immunoaffinity (IA) kits (including magnetic and non-magnetic versions of the
Streptavidin kit, Protein A kit, and Protein G kit), which combine the IA capture
and digestion process into a single well. The assay is required to be both
selective and accurate.  

? The rapid processing of antibodies using the SMART
 Digest IA kits allows development of faster methods
 compared to conventional protein enrichment and
 digestion by simplifying sample preparation and
 enabling a reproducible, sensitive, and fully integrated
 LC/MS/MS workflow.

? This application shows a rapid, simple, and sensitive
 method for the quantification of human IgG in mouse
 plasma using the SMART Digest IA kits with a total time
 of 3–4 hours that compares to a process that can take
 up to 24 hours.

? The method described provides a significant
 simplification for the affinity proteomics process within
 a single well providing increased sensitivity and
 reduced sample preparation time.

? This Application Note provides a general approach for
 quantifying low abundance mAbs with reproducible
 results across a broad dynamic range for all three types
 of SMART Digest (IA) kits (SMART digest IA
 Streptavidin, Protein A, and Protein G).

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