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  • 应用领域:生物产业
  • 发布时间:2016-07-21
  • 检测样品:代谢组学
  • 检测项目:代谢组学系统研究
  • 浏览次数:181 次
  • 下载次数:17 次
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Metabolomics allows researchers to interrogate thousands of potential biomarkers simultaneously, without a priori knowledge of the underlying biology or pathophysiology. Due to the complexity of the samples, people need outstanding mass resolution, mass accuracy, and sensitivity as well as sophisticated data analysis software. Agilent 6545 Q - TOF LC/MS system and Agilent MassHunter software are the perfect combination to fulfill the requirements. In this application, serum extracts from healthy subjects and samples with chronic kidney disease (CKD) at stage 3 and stage 5 are analyzed with 6545 LC/Q - TOF and 1290 Infinity LC system. The mass s pectrometer is tuned using the novel Swarm Autotune for optimal performance in the m/z range of the metabolites. Data was acquired in both positive and negative ionization modes with reference mass correction. The typical metabolomics workflow is ill ustrated here. Big sensitivity gains improve results The improved sensitivity of the 6545 Q - TOF LC/MS enables us to extract 5,817 features as opposed to 2,102 features on the previous generation of Q - TOF under the same column load in positive ionization mode. Even with a five - fold reduction of column load, we are still able to extract 2,489 features.
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